Our Publications

2026

• Valt M, Pánek T, Mirzoyan S, Tice AK, Jones RE, Dohnálek V, Doležal P, Mikšátko J, Rotterová J, Hrubá P, Brown MW, Čepička I. Rare microbial relict sheds light on an ancient eukaryotic supergroup. Nature, 649(8096), 388–395. (2026)
  DOI: 10.1038/s41586-025-09750-0

2025

• Janowicz N, Dohnálek V, Zítek J, Peña-Diaz P, Pyrihová E, King MS, Husová M, Žárský V, Kunji E, Zikova A, Hampl V, Dolezal P. Selective loss of ATP carriers in favour of SLC25A43 orthologues in metamonad mitochondria adapted to anaerobiosis. Open Biol, 15(8), 240202. (2025)
  DOI: 10.1098/rsob.240202
• Malych R, Folgosa F, Pilátová J, Mikeš L, Dohnálek V, Mach J, Matějková M, Kopecký V, Doležal P, Sutak R. Eating the brain - A multidisciplinary study provides new insights into the mechanisms underlying the cytopathogenicity of Naegleria fowleri. PLoS Pathog, 21(3), e1012995. (2025)
  DOI: 10.1371/journal.ppat.1012995

2024

• Dohnálek V, Doležal P. Installation of LYRM proteins in early eukaryotes to regulate the metabolic capacity of the emerging mitochondrion. Open Biol, 14(5), 240021. (2024)
  DOI: 10.1098/rsob.240021
• Richards TA, Eme L, Archibald JM, Leonard G, Coelho SM, de Mendoza A, Dessimoz C, Dolezal P, Fritz-Laylin LK, Gabaldón T, Hampl V, Kops GJPL, Leger MM, Lopez-Garcia P, McInerney JO, Moreira D, Muñoz-Gómez SA, Richter DJ, Ruiz-Trillo I, Santoro AE, Sebé-Pedrós A, Snel B, Stairs CW, Tromer EC, van Hooff JJE, Wickstead B, Williams TA, Roger AJ, Dacks JB, Wideman JG. Reconstructing the last common ancestor of all eukaryotes. PLoS Biol, 22(11), e3002917. (2024)
  DOI: 10.1371/journal.pbio.3002917
• Simoes-Barbosa A, McRae KM, Waghorn TS, Dolezal P, Ralston KS, Bilic I, Hirt RP, Sullivan SA, Nozaki T. ICAP 2024: anaerobic protozoology in focus, a partnership with parasitology societies in the South Pacific. Trends Parasitol, 40(11), 935–941. (2024)
  DOI: 10.1016/j.pt.2024.09.011
• Tushir S, Jhanwar P, Benda M, Horáčková V, Doležal P, Tatu U. In vivo Validation of Hsp90 Trans-splicing in Giardia lamblia: Highlighting the Role of Cis-elements. J Mol Biol, 436(4), 168440. (2024)
  DOI: 10.1016/j.jmb.2024.168440
• Vinopalová M, Arbonová L, Füssy Z, Dohnálek V, Samad A, Bílý T, Vancová M, Doležal P. Mlf mediates proteotoxic response via formation of cellular foci for protein folding and degradation in Giardia. PLoS Pathog, 20(10), e1012617. (2024)
  DOI: 10.1371/journal.ppat.1012617

2023

• Genge MG, Roy Chowdhury S, Dohnálek V, Yunoki K, Hirashima T, Endo T, Doležal P, Mokranjac D. Two domains of Tim50 coordinate translocation of proteins across the two mitochondrial membranes. Life Sci Alliance, 6(12), e202302122. (2023)
  DOI: 10.26508/lsa.202302122
• Motyčková A, Voleman L, Najdrová V, Arbonová L, Benda M, Dohnálek V, Janowicz N, Malych R, Šuťák R, Ettema TJG, Svärd S, Stairs CW, Doležal P. Adaptation of the late ISC pathway in the anaerobic mitochondrial organelles of Giardia intestinalis. PLoS Pathog, 19(10), e1010773. (2023)
  DOI: 10.1371/journal.ppat.1010773
• Shaliutina-Loginova A, Francetic O, Doležal P. Bacterial Type II Secretion System and Its Mitochondrial Counterpart. mBio, 14(2), e0314522. (2023)
  DOI: 10.1128/mbio.03145-22

2022

• Fry MY, Najdrová V, Maggiolo AO, Saladi SM, Doležal P, Clemons WM Jr. Structurally derived universal mechanism for the catalytic cycle of the tail-anchored targeting factor Get3. Nat Struct Mol Biol, 29(8), 820–830. (2022)
  DOI: 10.1038/s41594-022-00798-4
• Horáčková V, Voleman L, Hagen KD, Petrů M, Vinopalová M, Weisz F, Janowicz N, Marková L, Motyčková A, Najdrová V, Tůmová P, Dawson SC, Doležal P. Efficient CRISPR/Cas9-mediated gene disruption in the tetraploid protist Giardia intestinalis. Open Biol, 12(4), 210361. (2022)
  DOI: 10.1098/rsob.210361

2021

• Füssy Z, Vinopalová M, Treitli SC, Pánek T, Smejkalová P, Čepička I, Doležal P, Hampl V. Retortamonads from vertebrate hosts share features of anaerobic metabolism and pre-adaptations to parasitism with diplomonads. Parasitol Int, 82, 102308. (2021)
  DOI: 10.1016/j.parint.2021.102308
• Horváthová L, Žárský V, Pánek T, Derelle R, Pyrih J, Motyčková A, Klápšťová V, Vinopalová M, Marková L, Voleman L, Klimeš V, Petrů M, Vaitová Z, Čepička I, Hryzáková K, Harant K, Gray MW, Chami M, Guilvout I, Francetic O, Franz Lang B, Vlček Č, Tsaousis AD, Eliáš M, Doležal P. Analysis of diverse eukaryotes suggests the existence of an ancestral mitochondrial apparatus derived from the bacterial type II secretion system. Nat Commun, 12(1), 2947. (2021)
  DOI: 10.1038/s41467-021-23046-7
• Petrů M, Dohnálek V, Füssy Z, Doležal P. Fates of Sec, Tat, and YidC Translocases in Mitochondria and Other Eukaryotic Compartments. Mol Biol Evol, 38(12), 5241–5254. (2021)
  DOI: 10.1093/molbev/msab253
• Tůmová P, Voleman L, Klingl A, Nohýnková E, Wanner G, Doležal P. Inheritance of the reduced mitochondria of Giardia intestinalis is coupled to the flagellar maturation cycle. BMC Biol, 19(1), 193. (2021)
  DOI: 10.1186/s12915-021-01129-7

2020

• Najdrová V, Stairs CW, Vinopalová M, Voleman L, Doležal P. The evolution of the Puf superfamily of proteins across the tree of eukaryotes. BMC Biol, 18(1), 77. (2020)
  DOI: 10.1186/s12915-020-00814-3

2019

• Voleman L, Doležal P. Mitochondrial dynamics in parasitic protists. PLoS Pathog, 15(11), e1008008. (2019)
  DOI: 10.1371/journal.ppat.1008008

2018

• Petrů M, Wideman J, Moore K, Alcock F, Palmer T, Doležal P. Evolution of mitochondrial TAT translocases illustrates the loss of bacterial protein transport machines in mitochondria. BMC Biol, 16(1), 141. (2018)
  DOI: 10.1186/s12915-018-0607-3
• Pyrihová E, Motycková A, Voleman L, Wandyszewska N, Fišer R, Seydlová G, Roger A, Kolísko M, Doležal P. A Single Tim Translocase in the Mitosomes of Giardia intestinalis Illustrates Convergence of Protein Import Machines in Anaerobic Eukaryotes. Genome Biol Evol, 10(10), 2813–2822. (2018)
  DOI: 10.1093/gbe/evy215

2017

• Voleman L, Najdrová V, Ástvaldsson Á, Tůmová P, Einarsson E, Švindrych Z, Hagen GM, Tachezy J, Svärd SG, Doležal P. Giardia intestinalis mitosomes undergo synchronized fission but not fusion and are constitutively associated with the endoplasmic reticulum. BMC Biol, 15(1), 27. (2017)
  DOI: 10.1186/s12915-017-0361-y

2016

• Karnkowska A, Vacek V, Zubáčová Z, Treitli SC, Petrželková R, Eme L, Novák L, Žárský V, Barlow LD, Herman EK, Soukal P, Hroudová M, Doležal P, Stairs CW, Roger AJ, Eliáš M, Dacks JB, Vlček Č, Hampl V. A Eukaryote without a Mitochondrial Organelle. Curr Biol, 26(10), 1274–84. (2016)
  DOI: 10.1016/j.cub.2016.03.053
• Pyrih J, Pyrihová E, Kolísko M, Stojanovová D, Basu S, Harant K, Haindrich AC, Doležal P, Lukeš J, Roger A, Tachezy J. Minimal cytosolic iron-sulfur cluster assembly machinery of Giardia intestinalis is partially associated with mitosomes. Mol Microbiol, 102(4), 701–714. (2016)
  DOI: 10.1111/mmi.13487
• Žárský V, Doležal P. Evolution of the Tim17 protein family. Biol Direct, 11(1), 54. (2016)
  DOI: 10.1186/s13062-016-0157-y

2015

• King NP, Newton P, Schuelein R, Brown DL, Petru M, Zarsky V, Dolezal P, Luo L, Bugarcic A, Stanley AC, Murray RZ, Collins BM, Teasdale RD, Hartland EL, Stow JL. Soluble NSF attachment protein receptor molecular mimicry by a Legionella pneumophila Dot/Icm effector. Cell Microbiol, 17(6), 767–84. (2015)
  DOI: 10.1111/cmi.12405
• Leger MM, Petrů M, Žárský V, Eme L, Vlček Č, Harding T, Lang BF, Eliáš M, Doležal P, Roger AJ. An ancestral bacterial division system is widespread in eukaryotic mitochondria. Proc Natl Acad Sci U S A, 112(33), 10239–46. (2015)
  DOI: 10.1073/pnas.1421392112
• Martincová E, Voleman L, Pyrih J, Žárský V, Vondráčková P, Kolísko M, Tachezy J, Doležal P. Probing the Biology of Giardia intestinalis Mitosomes Using In Vivo Enzymatic Tagging. Mol Cell Biol, 35(16), 2864–74. (2015)
  DOI: 10.1128/MCB.00448-15

2013

• Zubáčová Z, Novák L, Bublíková J, Vacek V, Fousek J, Rídl J, Tachezy J, Doležal P, Vlček C, Hampl V. The mitochondrion-like organelle of Trimastix pyriformis contains the complete glycine cleavage system. PLoS One, 8(3), e55417. (2013)
  DOI: 10.1371/journal.pone.0055417

2012

• Alcock F, Webb CT, Dolezal P, Hewitt V, Shingu-Vasquez M, Likić VA, Traven A, Lithgow T. A small Tim homohexamer in the relict mitochondrion of Cryptosporidium. Mol Biol Evol, 29(1), 113–22. (2012)
  DOI: 10.1093/molbev/msr165
• Dolezal P, Aili M, Tong J, Jiang JH, Marobbio CM, Lee SF, Schuelein R, Belluzzo S, Binova E, Mousnier A, Frankel G, Giannuzzi G, Palmieri F, Gabriel K, Naderer T, Hartland EL, Lithgow T. Legionella pneumophila secretes a mitochondrial carrier protein during infection. PLoS Pathog, 8(1), e1002459. (2012)
  DOI: 10.1371/journal.ppat.1002459
• Martincová E, Voleman L, Najdrová V, De Napoli M, Eshar S, Gualdron M, Hopp CS, Sanin DE, Tembo DL, Van Tyne D, Walker D, Marcinčiková M, Tachezy J, Doležal P. Live imaging of mitosomes and hydrogenosomes by HaloTag technology. PLoS One, 7(4), e36314. (2012)
  DOI: 10.1371/journal.pone.0036314
• Sutak R, Hrdy I, Dolezal P, Cabala R, Sedinová M, Lewin J, Harant K, Müller M, Tachezy J. Secondary alcohol dehydrogenase catalyzes the reduction of exogenous acetone to 2-propanol in Trichomonas vaginalis. FEBS J, 279(15), 2768–80. (2012)
  DOI: 10.1111/j.1742-4658.2012.08661.x
• Zarsky V, Tachezy J, Dolezal P. Tom40 is likely common to all mitochondria. Curr Biol, 22(12), R479–81. (2012)
  DOI: 10.1016/j.cub.2012.03.057

2011

• Gebert N, Gebert M, Oeljeklaus S, von der Malsburg K, Stroud DA, Kulawiak B, Wirth C, Zahedi RP, Dolezal P, Wiese S, Simon O, Schulze-Specking A, Truscott KN, Sickmann A, Rehling P, Guiard B, Hunte C, Warscheid B, van der Laan M, Pfanner N, Wiedemann N. Dual function of Sdh3 in the respiratory chain and TIM22 protein translocase of the mitochondrial inner membrane. Mol Cell, 44(5), 811–8. (2011)
  DOI: 10.1016/j.molcel.2011.09.025
• Jedelský PL, Doležal P, Rada P, Pyrih J, Smíd O, Hrdý I, Sedinová M, Marcinčiková M, Voleman L, Perry AJ, Beltrán NC, Lithgow T, Tachezy J. The minimal proteome in the reduced mitochondrion of the parasitic protist Giardia intestinalis. PLoS One, 6(2), e17285. (2011)
  DOI: 10.1371/journal.pone.0017285
• Rada P, Doležal P, Jedelský PL, Bursac D, Perry AJ, Šedinová M, Smíšková K, Novotný M, Beltrán NC, Hrdý I, Lithgow T, Tachezy J. The core components of organelle biogenesis and membrane transport in the hydrogenosomes of Trichomonas vaginalis. PLoS One, 6(9), e24428. (2011)
  DOI: 10.1371/journal.pone.0024428
• Tong J, Dolezal P, Selkrig J, Crawford S, Simpson AG, Noinaj N, Buchanan SK, Gabriel K, Lithgow T. Ancestral and derived protein import pathways in the mitochondrion of Reclinomonas americana. Mol Biol Evol, 28(5), 1581–91. (2011)
  DOI: 10.1093/molbev/msq305

2010

• Dolezal P, Dagley MJ, Kono M, Wolynec P, Likić VA, Foo JH, Sedinová M, Tachezy J, Bachmann A, Bruchhaus I, Lithgow T. The essentials of protein import in the degenerate mitochondrion of Entamoeba histolytica. PLoS Pathog, 6(3), e1000812. (2010)
  DOI: 10.1371/journal.ppat.1000812
• Likic VA, Dolezal P, Celik N, Dagley M, Lithgow T. Using hidden markov models to discover new protein transport machines. Methods Mol Biol, 619, 271–84. (2010)
  DOI: 10.1007/978-1-60327-412-8_16

2009

• Dagley MJ, Dolezal P, Likic VA, Smid O, Purcell AW, Buchanan SK, Tachezy J, Lithgow T. The protein import channel in the outer mitosomal membrane of Giardia intestinalis. Mol Biol Evol, 26(9), 1941–7. (2009)
  DOI: 10.1093/molbev/msp117
• Rada P, Smíd O, Sutak R, Dolezal P, Pyrih J, Zársky V, Montagne JJ, Hrdy I, Camadro JM, Tachezy J. The monothiol single-domain glutaredoxin is conserved in the highly reduced mitochondria of Giardia intestinalis. Eukaryot Cell, 8(10), 1584–91. (2009)
  DOI: 10.1128/EC.00181-09

2008

• Gatsos X, Perry AJ, Anwari K, Dolezal P, Wolynec PP, Likić VA, Purcell AW, Buchanan SK, Lithgow T. Protein secretion and outer membrane assembly in Alphaproteobacteria. FEMS Microbiol Rev, 32(6), 995–1009. (2008)
  DOI: 10.1111/j.1574-6976.2008.00130.x

2007

• Carlton JM, Hirt RP, Silva JC, Delcher AL, Schatz M, Zhao Q, Wortman JR, Bidwell SL, Alsmark UC, Besteiro S, Sicheritz-Ponten T, Noel CJ, Dacks JB, Foster PG, Simillion C, Van de Peer Y, Miranda-Saavedra D, Barton GJ, Westrop GD, Müller S, Dessi D, Fiori PL, Ren Q, Paulsen I, Zhang H, Bastida-Corcuera FD, Simoes-Barbosa A, Brown MT, Hayes RD, Mukherjee M, Okumura CY, Schneider R, Smith AJ, Vanacova S, Villalvazo M, Haas BJ, Pertea M, Feldblyum TV, Utterback TR, Shu CL, Osoegawa K, de Jong PJ, Hrdy I, Horvathova L, Zubacova Z, Dolezal P, Malik SB, Logsdon JM Jr, Henze K, Gupta A, Wang CC, Dunne RL, Upcroft JA, Upcroft P, White O, Salzberg SL, Tang P, Chiu CH, Lee YS, Embley TM, Coombs GH, Mottram JC, Tachezy J, Fraser-Liggett CM, Johnson PJ. Draft genome sequence of the sexually transmitted pathogen Trichomonas vaginalis. Science, 315(5809), 207–12. (2007)
  DOI: 10.1126/science.1132894
• Dolezal P, Dancis A, Lesuisse E, Sutak R, Hrdý I, Embley TM, Tachezy J. Frataxin, a conserved mitochondrial protein, in the hydrogenosome of Trichomonas vaginalis. Eukaryot Cell, 6(8), 1431–8. (2007)
  DOI: 10.1128/EC.00027-07
• Gentle IE, Perry AJ, Alcock FH, Likić VA, Dolezal P, Ng ET, Purcell AW, McConnville M, Naderer T, Chanez AL, Charrière F, Aschinger C, Schneider A, Tokatlidis K, Lithgow T. Conserved motifs reveal details of ancestry and structure in the small TIM chaperones of the mitochondrial intermembrane space. Mol Biol Evol, 24(5), 1149–60. (2007)
  DOI: 10.1093/molbev/msm031

2006

• Dolezal P, Likic V, Tachezy J, Lithgow T. Evolution of the molecular machines for protein import into mitochondria. Science, 313(5785), 314–8. (2006)
  DOI: 10.1126/science.1127895
• Pütz S, Dolezal P, Gelius-Dietrich G, Bohacova L, Tachezy J, Henze K. Fe-hydrogenase maturases in the hydrogenosomes of Trichomonas vaginalis. Eukaryot Cell, 5(3), 579–86. (2006)
  DOI: 10.1128/EC.5.3.579-586.2006

2005

• Dolezal P, Smíd O, Rada P, Zubácová Z, Bursać D, Suták R, Nebesárová J, Lithgow T, Tachezy J. Giardia mitosomes and trichomonad hydrogenosomes share a common mode of protein targeting. Proc Natl Acad Sci U S A, 102(31), 10924–9. (2005)
  DOI: 10.1073/pnas.0500349102

2004

• Dolezal P, Vanácová S, Tachezy J, Hrdý I. Malic enzymes of Trichomonas vaginalis: two enzyme families, two distinct origins. Gene, 329, 81–92. (2004)
  DOI: 10.1016/j.gene.2003.12.022
• Hrdy I, Hirt RP, Dolezal P, Bardonová L, Foster PG, Tachezy J, Embley TM. Trichomonas hydrogenosomes contain the NADH dehydrogenase module of mitochondrial complex I. Nature, 432(7017), 618–22. (2004)
  DOI: 10.1038/nature03149
• Sutak R, Dolezal P, Fiumera HL, Hrdy I, Dancis A, Delgadillo-Correa M, Johnson PJ, Müller M, Tachezy J. Mitochondrial-type assembly of FeS centers in the hydrogenosomes of the amitochondriate eukaryote Trichomonas vaginalis. Proc Natl Acad Sci U S A, 101(28), 10368–73. (2004)
  DOI: 10.1073/pnas.0401319101

2003

• Suchan P, Vyoral D, Petrák J, Šut'ák R, Rasoloson D, Nohýnková E, Doležal P, Tachezy J. Incorporation of iron into Tritrichomonas foetus cell compartments reveals ferredoxin as a major iron-binding protein in hydrogenosomes. Microbiology (Reading), 149(Pt 7), 1911–1921. (2003)
  DOI: 10.1099/mic.0.26122-0